Detection and inhibition of Clostridium botulinum in some Egyptian fish products by probiotics cell-free supernatants as bio-preservation agents

The detection and inhibition of Clostridium botulinum by probiotics' cell-free supernatants, as a bio-preservation strategy, were investigated in some Egyptian fish-stuffs i.e., canned sardines, fesikh, moloha, renga, anchovy. conventional multiplex polymerase chain reaction used to detect C. botulinum, while the inhibitory antioxidant capacity four supernatants assessed using disc diffusion assay (DDA) radical scavenging activity approaches, respectively. results revealed that was isolated from 41.6% examined products, where type E. most predominant genotyping. DDA showed Lactobacillus bulgaricus EMCC 1102 L. paracasei 1103 expressed highest action against Meanwhile, has higher vitro (IC50) than 1103, mostly due its high bioactive constituent. Consequently, control growth E stored anchovy fillets. supernatant dose-dependent effect on These data provided an innovative perspective potential probiotics, guarantee fish products safety for further applications food industry.